T7 RNAP

    Low dsRNA T7 RNA Polymerase Mutants Empowering mRNA Vaccine and Therapy Development

Results Analysis:

1. ELISA for dsRNA content shows that mutants M1-5 have lower dsRNA levels, with M5 reducing by 98% compared to WT and 78% compared to a competitor (Fig 3- a).

2.   Dot blot analysis confirms that mutants M1-5 have reduced

dsRNA content (Fig 3-b).