T4 DNA Ligase

   Engineered T4 DNA Ligase with Enhanced Stability and low Adapter-Adapter Ligation

1. By protein engineering, the stability of T4 DNA ligase mutants increased significantly without sacrificing their activity. M6 could keep its activity 100% under 45 degree(Fig 2).

2. The Qseq tests showed that the adapter-adapter ligation ratio of the mutants decreased from 10% of the wild type to about 3%, and even lower to 1% of M1 and M4(Fig 3).